Why we should consider RNA-based NGS to identify NTRK gene
Both RNA-based and DNA-based NGS are capable of multiplexed assessment
for different fusion targets. However, RNA-based NGS is preferred for
identifying NTRK gene fusions, due to its capability to detect expressed
fusion transcripts directly, with more accurate breakpoint readout.
RNA-based NGS avoids the difficulties of sequencing larger intronic
regions associated with NTRK gene fusions. It also has a faster
sequencing process compare to DNA-based NGS. Its detection accuracy can
vary depending on the choice of library preparation methods, for
example, hybridization-based capture or amplicon-based approach.